LOCUS       V005229                 5787 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V005229
VERSION     V005229
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5787)
  AUTHORS   Toda H, Koyanagi T, Enomoto T, Itoh N.
  TITLE     Characterization of two cryptic plasmids from Kocuria palustris
            IPUFS-1 and construction of novel Escherichia coli-Kocuria shuttle
            vector for biocatalysis
  JOURNAL   J. Biosci. Bioeng. 124 (3), 255-262 (2017)
   PUBMED   28495560
REFERENCE   2  (bases 1 to 5787)
  AUTHORS   Itoh N, Toda H, Koyanagi T, Enomoto T.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2016) Contact:Nobuya Itoh Toyama Prefectural
            University, Biotechnology Research Center; 5180 Kurokawa, Imizu,
            Toyama 939-0398, Japan URL :http://www.pu-toyama.ac.jp/BR/itoh/
REFERENCE   3  (bases 1 to 5787)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5787)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. Biosci.
            Bioeng."; date: "2017"; volume: "124"; issue: "3"; pages: "255-262"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (12-DEC-2016) Contact:Nobuya Itoh Toyama Prefectural University,
            Biotechnology Research Center"; volume: " 5180 Kurokawa, Imizu,
            Toyama 939-0398, Japan URL :http"; pages:
            "//www.pu-toyama.ac.jp/BR/itoh"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5787
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          4175..5781
                     /plasmid="pKPAL1"
                     /strain="IPUFS-1"
                     /mol_type="other DNA"
                     /db_xref="taxon:71999"
                     /organism="Kocuria palustris"
     misc_feature    336..392
                     /label="MCS"
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(396..412)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      625..1080
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        1362..1466
                     /label="AmpR promoter"
     CDS             1467..2324
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      2498..3086
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             3314..4120
                     /gene="tsnR"
                     /label="23S rRNA (adenosine(1067)-2'-O)-methyltransferase"
                     /note="23S rRNA (adenosine(1067)-2'-O)-methyltransferase
                     from Streptomyces azureus. Accession#: P18644"
     CDS             4307..5182
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein"
                     /label="repA"
                     /protein_id="BAX51307.1"
                     /translation="MTTAECVQRWDQMWLPLWPLASDDLHAGVYRQARGEAMHRRYIET
                     NPRALSNLLVVDIDHEDAALRTMWNRQAWWPNAVVENPSNGHAHAVWALNEPITRTEYA
                     RRKPLAFAAAVTEGLRRSVDGDTGYSGLLTKNPAHDDWDALWCNSDRLYDLDELAEHLT
                     EGGYMPPVSWKRSKRRNSVGLGRNCSIFETARTWAYREIRNHWFDSAGLHDAISAHVHE
                     LNAEFPEPLPASEARAIAASIHRWITTKSRMWADGPAVYEATFSTIQAARGRKRVERTK
                     ALLEGWQPNG"
     gene            4307..5182
                     /gene="repA"
                     /label="repA"
     CDS             5175..5483
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein"
                     /label="repB"
                     /protein_id="BAX51308.1"
                     /translation="MGDRYDRKGSSISEMSRGTGLSPATIKRWTSRSRDEWLQQKADER
                     EAIRAFHDDEGHSWPQTAKHFRLDVSTVKRRAYRAREERKQEIAEQLQPPLPFPTNS"
     gene            5175..5483
                     /gene="repB"
                     /label="repB"
ORIGIN
        1 ccgaccgccg ggccgaggag ctggggatcg ccaccaccac cgagttcgcg gcccaggccg
       61 gaggggcgca ggcgcgggcc cactagcacg ggccggtgaa ccccgcatga atgttggttc
      121 atgtggggtt tgcgccagct gtgtcgcagt tgccccccgc cactgttccc ggggcatgaa
      181 tcccgctaga atgaacacgg aagagcaggt ggaccccggg tcgtcaccgc acttcgggtg
      241 tgggatttat gccgattggt tttgatccca catccggacg ggccggagtg caccgcacac
      301 ccactcgcag aggagattca tatgaccatg attacgaatt cgagctcggt acccggggat
      361 cctctagagt cgacctgcag gcatgcaagc ttggcactgg ccgtcgtttt acaacgtcgt
      421 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc
      481 agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
      541 aatggcgaat ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac
      601 cgcatacgtc aaagcaacca tagtacgcgc cctgtagcgg cgcattaagc gcggcgggtg
      661 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
      721 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
      781 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
      841 tgggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
      901 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
      961 tctcgggcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     1021 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg tttacaattt
     1081 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc
     1141 cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac
     1201 aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac
     1261 gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa
     1321 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     1381 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     1441 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     1501 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     1561 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     1621 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     1681 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     1741 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     1801 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     1861 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     1921 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     1981 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     2041 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     2101 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     2161 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     2221 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     2281 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     2341 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     2401 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     2461 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     2521 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     2581 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     2641 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     2701 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     2761 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     2821 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     2881 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     2941 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     3001 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     3061 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggcctttaga tcctgaactt
     3121 ttgctttgcc acggaacggt ctgcgttgtc gggaagatgc gtgatctgat ccttcaactc
     3181 agcaaaagtt cgatttattc aacaaagcca cgttgtgtct caaaatctct gatgttacat
     3241 tgcacaagat aaaaatatat catcatgaac aataaaactg tctgcttaca taacccgttg
     3301 gaggaggctc caaatgaccg agctggacac catcgcgaac ccctccgatc ccgccgtgca
     3361 gcggatcatc gacgtgacca aaccctcccg ctcgaacatc aagaccaccc tcatcgagga
     3421 cgtcgagccc ctgatgcact ccatcgccgc gggcgtggag ttcattgagg tgtacgggtc
     3481 cgactcgtcc ccgttcccct cggaactgct cgatctgtgc ggccgccaga acatccccgt
     3541 gcggctgatc gactcctcca tcgtgaacca gctcttcaag ggcgagcgca aagccaagac
     3601 cttcgggatc gcccgcgtgc cccgccccgc gcgcttcggc gacattgcct cccgccgcgg
     3661 cgacgtggtg gtgctggacg gcgtgaaaat cgtgggcaac atcggcgcga tcgtgcgcac
     3721 gtccctcgcc ctcggcgcct ccggcatcat cctggtggac tccgacatta cgtccatcgc
     3781 ggaccgccgc ctgcagcgcg cctcccgcgg gtatgtgttc tccctgcccg tggtgctgtc
     3841 cgggcgcgag gaggcgatcg ccttcatccg cgactccggc atgcagctga tgaccctgaa
     3901 ggccgacggc gacatctcgg tgaaggagct gggcgacaac cccgaccgcc tggcgctgct
     3961 gttcggctcc gagaagggcg gcccctccga cctgtttgag gaggcctcct ccgcgtccgt
     4021 gtccatcccc atgatgtccc agaccgagtc cctgaacgtg tccgtgtccc tgggcatcgc
     4081 gctgcacgag cgcatcgacc gcaacctcgc cgcgaaccgc tgagcgcctc tgttcctcgg
     4141 acgctcggtt gctcgacctc gattcgtcag atcttgcggc tgaacgaaca cgggcacccc
     4201 aggggcaaag cggactgcgc tgcgcgcggg tgggtggatg cccgaaacat ctgacttggc
     4261 taagagccac tttgcaccgg gtcatgtttg tgggagagtc gcacacatga cgacggcgga
     4321 gtgcgtgcag cggtgggatc agatgtggtt gccgctgtgg ccgttggcct ccgacgacct
     4381 gcacgcgggt gtgtaccggc aggcgcgcgg tgaggcgatg catcgtcgct acatcgagac
     4441 gaacccgcgt gcgctgagca acctgctcgt ggtggacatc gaccacgagg acgccgcgct
     4501 gcgcacgatg tggaaccgac aggcctggtg gcccaacgcg gtcgtggaga acccgtccaa
     4561 cggccacgcg cacgccgtct gggcgctcaa cgaaccgatc acccgcacgg agtacgcccg
     4621 tcgcaagccg ctggcgttcg cagcagctgt gaccgagggg ctgcgccgct ccgtggacgg
     4681 cgacacgggc tactccgggc tgctgaccaa gaaccccgca cacgacgact gggacgcgtt
     4741 gtggtgcaac agcgaccggc tctacgacct cgacgagctc gctgagcacc tcaccgaggg
     4801 cgggtacatg cctccggtgt cctggaagcg ctccaagcgc cgcaacagcg tcgggctggg
     4861 ccgcaactgc tcgatcttcg agaccgcacg cacctgggcc taccgcgaga tccgcaacca
     4921 ctggttcgac agtgccggcc tccacgacgc gatcagcgca cacgtgcacg aactcaatgc
     4981 cgagttcccc gaaccactgc cggcatcaga agcccgagcg atcgcggcca gcatccaccg
     5041 ctggatcacc accaagtccc gcatgtgggc cgacggcccc gccgtctacg aagccacgtt
     5101 ctccaccatc caagccgcac gcgggcgcaa gcgcgtggaa cgcaccaagg cactcttgga
     5161 ggggtggcag cccaatgggt gatcggtacg accgcaaggg gagttccatc tcggagatgt
     5221 cgcgaggaac tggtctgagc ccagccacga tcaagcgatg gacctcgcgt agtcgggatg
     5281 agtggcttca gcagaaagcc gacgagcgtg aggccatccg tgcgttccac gacgatgagg
     5341 ggcactcctg gccgcagacg gccaagcact tccggctcga cgtctcgacg gtgaagcgcc
     5401 gcgcgtatcg agcgcgtgag gagcgcaaac aggagatcgc cgagcagttg cagcctccgc
     5461 tgcccttccc caccaactcc tgaaccatca gctatcaaaa acgaaattcg cccccgggac
     5521 tccgacggag ccccgggggc gaatttccgg tatatggtat aatgtcctta gcgatcattc
     5581 caagcagtgc catgcctggg attccggtcg cccgaggaga tggtccatat ccgtagtgct
     5641 ctgtatatac attacgttat caggactgcg acatcagagc aaccgtggac tcccacgact
     5701 gccccgtcgc gacggcccaa gcgggccgcc cctaccgacc tcctctcctc ggaccttcgt
     5761 cgcgccgagg agaggaggaa cccatgg
//
